PRP & Autologous Biologics in Women’s Health & Aesthetics

Due to its ability to induce collagen synthesis by dermal fibroblasts, Platelet Rich Plasma has been proposed as a promising treatment option in the field of skin care (Abuaf et al. 2016, Cho et al. 2010, Cho et al. 2012, Kim et al. 2011). For a few years, it is successfully used in numerous dermal applications, including alopecia, scar revision (keloid or traumatic scars), acne scars, stretch marks, skin rejuvenation (overall improvement in skin texture and firmness) and dermal augmentation. 

On another hand, combination of PRP and hyaluronic acid (HA) is also used in the field of skin care for intra-dermal injections, for the hydration of dehydrated and wrinkled skin. It has also been shown that this combination with HA prolong the effects of PRP, thereby improving skin tone and quality (Hersant et al. 2017, chapter 4).

Topics discussed:

I) PRP biology in tissue healing, skin rejuvenation and alopecia: (1h-1h30min)

• Role of plasma and platelet in skin rejuvenation and atrophic scars

1. Definition, role of platelet and plasma in dermal applications
2. Importance of having a high quality PRP, instruction for use (reproductibility of the procedure, ensure Red Blood Cell depletion, good practice of use and traceability).

• Combination of PRP and Autologous Thrombin as a new treatment option in aesthetics

1. How to extract thrombin and how to mix it, in good proportion, with A-PRP
2. Autologous alternative to classic treatment option for peri-orbital area (discoloration of the julgo-palpebral fissure…)

• Involvement of A-PRP in alopecia

1. Evidence of A-PRP efficacy on acute and chronic AA
2. Involvment of A-PRP during hair transplant procedures

• Dermal and alopecia applications

1. Area to treat: peri-orbital area, superficial wrinkles, tear trough, decolete, neck
2. Alopecia applications

II) Involvement of A-PRP in fibroblast and Adipose Derived Mesenchymal Stem Cells proliferation (ADSC): impact of A-PRP in fat grafting (30min)

• Comparison between FBS media and autologous PRP media for fibroblast expansion

1. A-PRP dose-dependent proliferation effect on fibroblast expansion
2. Importance of having a non-activated PRP for fibroblast expansion

• A-PRP: A biological supplement to enhance ADSC expansion

1. An alternative to FBS media for clinical MSC expansion
2. Biosafety criterion respected with the use of non-activated A-PRP

III) Clinical assessments and Literature Search concerning A-PRP in dermal applications (30min)

• Clinical background, current knowledge, state of the art

1. Pathophysiology of skin aging and acne scars
2. Current therapeutic options

• Clinical relevance of A-PRP for the treatment of skin defects such as atrophic acne scar treatment

1. Examples of Clinical Cases
2. References

IV) Practical cases with different injections techniques (4-5h)

• Dermal and aesthetics injections techniques

1. Peri-orbital injections using a cannula for the combination of PRP and Autologous Thrombin (retro-tracing injections)
2. Injections of A-PRP in deep dermis (point by point)
3. Transdermal superficial injections of A-PRP in the face (micro-needling)

• Alopecia injections techniques

1. Manual micro-needling injection technique
2. Automatic micro-needling injection technique

- First hand knowledge with pioneering practitioners

- Multiple clinical cases with hands-on training

- Comfortable environment and best set-up for theoretical and practical learning

- Expert insights into PRP medical devices & preparations

M.D.

To be discussed in session.

At the end of this workshop, a BioBridge certificate of attendance will be provided.

The workshop will be divided in two parts.

The morning session will be dedicated to the theoretical part with the presentation of Platelet Rich Plasma (PRP) biology and medical devices by the product specialist.

In the afternoon session the guest doctor will make live injections on patients and will guide you through the hands-on training.

09.00 – 09.30 Welcome snack

Theoretical part

09.30 - 11.00

PRP biology in tissue healing, skin rejuvenation and alopecia

11.00 - 11.30

Involvement of A-PRP in fibroblast and Adipose Derived Mesenchymal Stem Cells proliferation (ADSC): impact of A-PRP in fat grafting

11.30 - 12.00

Clinical assessments and Literature Search concerning A-PRP in dermal applications

12.00 – 13.00 Gastronomic Lunch Break (included)

Live demonstration and hands-on training

13.00 – 17.00

Practical cases with different injections techniques

• Dermal and aesthetics injections techniques
• Alopecia injections techniques

17.00 – 17.15 Certificate of attendance handing-out

17.15 - 18.00 Drink